Propagating DNA in Bacteria

Recombinant DNA Technology

OSC Microbio 12 01 MolCloning

Gene cloning

Additional Resources:


Questions for thought

  1. Why do you think that origins of replication are made of A’s and T’s?
  2. What is different about the types of bonds holding the double strands together versus phosphodiester bonds of the DNA backbone?
  3. Can DNA digested with SmaI be ligated to DNA digested with EcoRV?
  4. If so, which enzyme will be able to digest this new DNA?